Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-AI (DE3) cells | Triticum turgidum subsp. durum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
propyl gallate | almost complete inhibition (99%) in the presence of 1 mM propyl gallate | Triticum turgidum subsp. durum | |
Salicylhydroxamic acid | 70% inhibition in the presence of 1 mM salicylhydroxamic acid | Triticum turgidum subsp. durum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07369 | - |
linoleic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum | |
0.3001 | - |
linolenic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum | |
0.741 | - |
arachidonic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum turgidum subsp. durum | A0A7M3TWE5 | durum wheat | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Triticum turgidum subsp. durum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Triticum turgidum subsp. durum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + O2 | very low activity and specific activity towards arachidonic acid | Triticum turgidum subsp. durum | ? | - |
? | |
linoleic acid + O2 | preferred substrate | Triticum turgidum subsp. durum | (10E,12Z)-13-hydroperoxy-10,12-octadecadienoate | - |
? | |
linolenic acid + O2 | good preference for linolenic acid | Triticum turgidum subsp. durum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 96750, calculated from amino acid sequence | Triticum turgidum subsp. durum |
Synonyms | Comment | Organism |
---|---|---|
13-LOX | - |
Triticum turgidum subsp. durum |
LOX2 | - |
Triticum turgidum subsp. durum |
Lpx-A2 | - |
Triticum turgidum subsp. durum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Triticum turgidum subsp. durum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum turgidum subsp. durum | calculated from amino acid sequence | - |
6.1 |
Organism | Comment | Expression |
---|---|---|
Triticum turgidum subsp. durum | the up-regulation of the enzyme gene is a component of the durum wheat response to hyperosmotic stress. Under mannitol and NaCl stress, a strong increase in the enzyme activity is observed | up |